Xaa-Pro aminopeptidase 1 (XPNPEP1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-623aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: PPKVTSELLR QLRQAMRNSE YVTEPIQAYI IPSGDAHQSE YIAPCDCRRA FVSGFDGSAG TAIITEEHAA MWTDGRYFLQ AAKQMDSNWT LMKMGLKDTP TQEDWLVSVL PEGSRVGVDP LIIPTDYWKK MAKVLRSAGH HLIPVKENLV DKIWTDRPER PCKPLLTLGL DYTGISWKDK VADLRLKMAE RNVMWFVVTA LDEIAWLFNL RGSDVEHNPV FFSYAIIGLE TIMLFIDGDR IDAPSVKEHL LLDLGLEAEY RIQVHPYKSI LSELKALCAD LSPREKVWVS DKASYAVSET IPKDHRCCMP YTPICIAKAV KNSAESEGMR RAHIKDAVAL CELFNWLEKE VPKGGVTEIS AADKAEEFRR QQADFVDLSF PTISSTGPNG AIIHYAPVPE TNRTLSLDEV YLIDSGAQYK DGTTDVTRTM HFGTPTAYEK ECFTYVLKGH IAVSAAVFPT GTKGHLLDSF ARSALWDSGL DYLHGTGHGV GSFLNVHEGP CGISYKTFSD EPLEAGMIVT DEPGYYEDGA FGIRIENVVL VVPVKTKYNF NNRGSLTFEP LTLVPIQTKM IDVDSLTDKE CDWLNNYHLT CRDVIGKELQ KQGRQEALEW LIRETQPISK QH. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Contributes to the degradation of bradykinin. Catalyzes the roval of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro. Reference: "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S. Nat. Genet. 36:40-45(2004). Function: Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro.