WDR77 (human, recombinant)

Methylosome protein WDR77, also known as methylosome protein 50 (MEP50) and androgen receptor cofactor p44, is an obligate cofactor of protein arginine methyltransferase 5 (PRMT5) and androgen receptor coactivator. It contains 342 amino acid residues and six putative WD-40 repeats that act as scaffolds for protein-protein interactions. WDR77 is ubiquitously expressed and localizes to both the cytoplasm and nucleus. It binds to PRMT5 to stabilize and increase its activity, as well as recruits substrates to the PRMT5 complex for methylation. WDR77 acts as a coactivator of androgen receptor transcription mediated through an androgen receptor-Smad1-WDR77 complex. High cytoplasmic and low nuclear levels of WDR77 are associated with the development of castration-resistant prostate cancer. Cayman's WDR77 (human, recombinant) protein has a calculated molecular weight of 68 kDa.