UGRP2, Recombinant, Human, aa21-104 (PnSP-2, HIN-1, High in Normal-1, SCGB3A1)

High in Normal-1 (HIN-1), also known as Uteroglobin related protein 2 (UGRP2), is an 8kD secreted protein of the secretoglobin superfamily (SCGB3A1) (1). HIN-1 is expressed in bronchial epithelial and secretory Clara cells, mammary epithelial cells (particularly during pregnancy), salivary glands, and the prostate (2-5). It is up-regulated in Clara cells by IL-4, IL-13, Oncostatin M, EGF, and TGF-alpha and down­regulated by IFN­alpha, beta, and gamma (6-9). It is secreted into bronchial lavage fluid, saliva, and plasma, and may form disulfide-linked dimers (10). HIN-1 binds to the macrophage scavenger receptor MARCO, and to the surface of mammary and bronchial epithelial cells (11, 12). HIN-1 promotes apoptosis and inhibits the proliferation, migration, and invasion of breast cancer cells (12). The down-regulation of HIN-1 expression in many breast, lung, and prostate cancers correlates with hypermethylation of its promoter (4, 10, 13, 14). Mature human HIN-1 shares 58% and 62% aa sequence identity with mouse and rat HIN-1, respectively (4). Source: Recombinant protein corresponding to Phe21-Gly104 from human HIN-1/SCGB3A1, expressed in E. coli. Molecular Weight: ~6kD, Endotoxin: <0.10EU/1ug (LAL method), Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells. The ED50 for this effect is 0.15-0.75ug/mL. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute in sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.