Twinkle protein, mitochondrial (PEO1), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 32-684aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: APGPPRRRYR KETLQALDMP VLPVTATEIR QYLRGHGIPF QDGHSCLRAL SPFAESSQLK GQTGVTTSFS LFIDKTTGHF LCMTSLAEGS WEDFQASVEG RGDGAREGFL LSKAPEFEDS EEVRRIWNRA IPLWELPDQE EVQLADTMFG LTKVTDDTLK RFSVRYLRPA RSLVFPWFSP GGSGLRGLKL LEAKCQGDGV SYEETTIPRP SAYHNLFGLP LISRRDAEVV LTSRELDSLA LNQSTGLPTL TLPRGTTCLP PALLPYLEQF RRIVFWLGDD LRSWEAAKLF ARKLNPKRCF LVRPGDQQPR PLEALNGGFN LSRILRTALP AWHKSIVSFR QLREEVLGEL SNVEQAAGLR WSRFPDLNRI LKGHRKGELT VFTGPTGSGK TTFISEYALD LCSQGVNTLW GSFEISNVRL ARVMLTQFAE GRLEDQLDKY DHWADRFEDL PLYFMTFHGQ QSIRTVIDTM QHAVYVYDIC HVIIDNLQFM MGHEQLSTDR IAAQDYIIGV FRKFATDNNC HVTLVIHPRK EDDDKELQTA SIFGSAKASQ EADNVLILQD RKLVTGPGKR YLQVSKNRFD GDVGVFPLEF NKNSLTFSIP PKNKARLKKI KDDTGPVAKK PSSGKKGATT QNSEICSGQA PTPDQPDTSK RSK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Involved in mitochondrial DNA (mtDNA) metabolism. Could function as an adenine nucleotide-dependent DNA helicase. Function inferred to be critical for lifetime maintenance of mtDNA integrity. In vitro, forms in combination with POLG, a processive replication machinery, which can use double-stranded DNA (dsDNA) as tplate to synthesize single-stranded DNA (ssDNA) molecules. May be a key regulator of mtDNA copy number in mammals. Reference: Human mitochondrial DNA deletions associated with mutations in the gene for Twinkle, a phage T7 gene 4-like protein localized in mitochondria.Spelbrink J.N., Li F.-Y., Tiranti V., Nikali K., Yuan Q.-P., Tariq M., Wanrooij S., Garrido N., Comi G., Morandi L., Santoro L., Toscano A., Fabrizi G.-M., Somer H., Croxen R., Beeson D., Poulton J., Suomalainen A. , Jacobs H.T., Zeviani M., Larsson C.Nat. Genet. 28:223-231(2001). Function: Involved in mitochondrial DNA (mtDNA) metabolism. Could function as an adenine nucleotide-dependent DNA helicase. Function inferred to be critical for lifetime maintenance of mtDNA integrity. In vitro, forms in combination with POLG, a processive replication machinery, which can use double-stranded DNA (dsDNA) as template to synthesize single-stranded DNA (ssDNA) molecules. May be a key regulator of mtDNA copy number in mammals.