Toxin zeta, Streptococcus agalactiae, recombinant

Organism: Streptococcus agalactiae. Source: Yeast. Expression Region: 1-287aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MANITDFTEK QFEDRLEKNV ERLTKNRLAV ESPTAFLLGG QPGSGKTSLR SAISEETQGN VVIIDNDTFK QQHPNFDELV KLYEKDVVKY VTPYSNRMTE AIISRLRDKG YNLVIEGTGR TTDVPIQTAT MLQAKDYETK MYVMAVPKIN SYLGTIERYE TMYADDPMTA RATPKQAHDI VVKNLPTNLE TLHKTGLFSD IRLYNREGVK LYSSLETPSI SPKETLEREL NRKVSGKEIQ PTLERIEQKM VQNQHQETPE FKAIQQKMES LQPPTPPIPK TPKLPGI. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Toxic component of a toxin-antitoxin (TA) module. Phosphorylates UDP-N-acetyl-D-glucosamine (UNAG) on the 3'-hydroxyl group of the N-acetyl-D-glucosamine moiety, yielding UNAG-3P. UNAG-3P inhibits MurA, the first committed step in cell wall synthesis, which is then blocked. Phosphorylation is inhibited by cognate epsilon antitoxin. Part of a postsegregational killing (PSK) syst involved in the killing of plasmid-free cells. The zeta toxin induces programmed cell death . Reference: Lactobacillus helveticus ATCC15009 as a host for native and conjugative plasmid DNA.Thompson J.K., Collins M.A., Foley S. Function: Toxic component of a type II toxin-antitoxin (TA) system. Phosphorylates UDP-N-acetyl-D-glucosamine (UNAG) on the 3'-hydroxyl group of the N-acetyl-D-glucosamine moiety, yielding UNAG-3P. UNAG-3P inhibits MurA, the first committed step in cell wall synthesis, which is then blocked. Phosphorylation is inhibited by cognate epsilon antitoxin. Part of a postsegregational killing (PSK) system involved in the killing of plasmid-free cells. The zeta toxin induces programmed cell death (By similarity).