Toll-like receptor 4 (Tlr4), partial, rat, recombinant

Organism: Rattus norvegicus (Rat). Source: in vitro E.coli expression system. Expression Region: 26-638aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. Target Protein Sequence: NPCIEVLPNI TYQCMDQNLS KIPHDIPYST KNLDLSFNPL KILRSYSFTN FSQLQWLDLS RCEIETIEDK AWHGLNQLST LVLTGNPIKS FSPGSFSGLT NLENLVAVET KMTSLEGFHI GQLISLKKLN VAHNLIHSFK LPEYFSNLTN LEHVDLSYNY IQTISVKDLQ FLRENPQVNL SLDLSLNPID SIQAQAFQGI RLHELTLRSN FNSSNVLKMC LQNMTGLHVH RLILGEFKNE RNLESFDRSV MEGLCNVSID EFRLTYINHF SDDIYNLNCL ANISAMSFTG VHIKHIADVP RHFKWQSLSI IRCHLKPFPK LSLPFLKSWT LTTNREDISF GQLALPSLRY LDLSRNAMSF RGCCSYSDFG TNNLKYLDLS FNGVILMSAN FMGLEELEYL DFQHSTLKKV TEFSVFLSLE KLLYLDISYT NTKIDFDGIF LGLISLNTLK MAGNSFKDNT LSNVFTNTTN LTFLDLSKCQ LEQISRGVFD TLYRLQLLNM SHNNLLFLDP SHYKQLYSLR TLDCSFNRIE TSKGILQHFP KSLAVFNLTN NSVACICEYQ NFLQWVKDQK MFLVNVEQMK CASPIDMKAS LVLDFTNSTC YIYKTIISVS VVS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL. Reference: "Toll-like receptor 4: the missing link of the cerebral innate immune response triggered by circulating gram-negative bacterial cell wall components."Laflamme N., Rivest S.FASEB J. 15:155-163(2001). Function: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (By similarity).