Thrombospondin-1 (Thbs1), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 19-350aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: NRIPESGGDN GVFDIFELIG GARRGPGRRL VKGQDLSSPA FRIENANLIP AVPDDKFQDL LDAVWADKGF IFLASLRQMK KTRGTLLAVE RKDNTGQIFS VVSNGKAGTL DLSLSLPGKQ QVVSVEEALL ATGQWKSITL FVQEDRAQLY IDCDKMESAE LDVPIQSIFT RDLASVARLR VAKGDVNDNF QGVLQNVRFV FGTTPEDILR NKGCSSSATN VLLTLDNNVV NGSSPAIRTN YIGHKTKDLQ AICGLSCDEL SSMVLELKGL RTIVTTLQDS IRKVTEENRE LVSELKRPPL CFHNGVQYKN NEEWTVDSCT ECHCQNSVTI CK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties . Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors.1 Publication. Reference: Characterization of the murine thrombospondin gene.Lawler J., Duquette M., Ferro P., Copeland N.G., Gilbert D.J., Jenkins N.A.Genomics 11:587-600(1991). Function: nan