Tetranectin (CLEC3B), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 22-202aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: EPPTQKPKKI VNAKKDVVNT KMFEELKSRL DTLAQEVALL KEQQALQTVC LKGTKVHMKC FLAFTQTKTF HEASEDCISR GGTLGTPQTG SENDALYEYL RQSVGNEAEI WLGLNDMAAE GTWVDMTGAR IAYKNWETEI TAQPDGGKTE NCAVLSGAAN GKWFDKRCRD QLPYICQFGI V. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis. Reference: "Structure of the plasminogen kringle 4 binding calcium-free form of the C-type lectin-like domain of tetranectin." Nielbo S., Thomsen J.K., Graversen J.H., Jensen P.H., Etzerodt M., Poulsen F.M., Thogersen H.C. Biochemistry 43:8636-8643(2004). Function: nan