Tetanus toxin, partial, Clostridium tetani, recombinant

Organism: Clostridium tetani. Source: E.coli. Expression Region: 1098-1310aa+EGWIN. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: NPKEIEKLYT SYLSITFLRD FWGNPLRYDT EYYLIPVAYS SKDVQLKNIT DYMYLTNAPS YTNGKLNIYY RRLYSGLKFI IKRYTPNNEI DSFVRSGDFI KLYVSYNNNE HIVGYPKDGN AFNNLDRILR VGYNAPGIPL YKKMEAVKLR DLKTYSVQLK LYDDKDASLG LVGTHNGQIG NDPNRDILIA SNWYFNHLKD KTLTCDWYFV PTDEGWIN. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Plays a role in formation of the lens suture in the eye, which is important for normal optical properties of the lens. Reference: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. Gray A.M. Genome Res. 13:2265-2270(2003). Function: nan