Talin-2 (TLN2), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 88-406aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: RPQKIRMLDG SVKTVMVDDS KTVGELLVTI CSRIGITNYE EYSLIQETIE EKKEEGTGTL KKDRTLLRDE RKMEKLKAKL HTDDDLNWLD HSRTFREQGV DENETLLLRR KFFYSDQNVD SRDPVQLNLL YVQARDDILN GSHPVSFEKA CEFGGFQAQI QFGPHVEHKH KPGFLDLKEF LPKEYIKQRG AEKRIFQEHK NCGEMSEIEA KVKYVKLARS LRTYGVSFFL VKEKMKGKNK LVPRLLGITK DSVMRVDEKT KEVLQEWPLT TVKRWAASPK SFTLDFGEYQ ESYYSVQTTE GEQISQLIAG YIDIILKKK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity . Reference: Recruitment and regulation of phosphatidylinositol phosphate kinase type 1 gamma by the FERM domain of talin.Di Paolo G., Pellegrini L., Letinic K., Cestra G., Zoncu R., Voronov S., Chang S., Guo J., Wenk M.R., De Camilli P.Nature 420:85-89(2002). Function: As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity (By similarity).