T-cell leukemia virus 1 HTLV-1 basic zipper factor (HBZ), human, recombinant

Organism: Human T-cell leukemia virus 1 (strain Japan ATK-1 subtype A) (HTLV-1). Source: E.coli. Expression Region: 1-209aa. Protein Length: Full length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MVNFVSAGLF RCLPVSCPED LLVEELVDGL LSLEEELKDK EEEEAVLDGL LSLEEESRGR LRRGPPGEKA PPRGETHRDR QRRAEEKRKR KKEREKEEEK QTAEYLKRKE EEKARRRRRA EKKAADVARR KQEEQERRER KWRQGAEKAK QHSARKEKMQ ELGIDGYTRQ LEGEVESLEA ERRKLLQEKE DLMGEVNYWQ GRLEAMWLQ. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Contributes to the regulation of viral RNA transcription by interacting with host proteins involved in transcriptional activation such as ATF4, or CREB1, and by inhibiting their activity. Additionally, HBZ suppresses host NF-kappa-B-driven transcription mediated by host RELA as well as transcription of some classical NF-kappa-B target genes, including IL8, IL2RA, IRF4, VCAM1, and VEGFA. Reference: "The HBZ factor of human T-cell leukemia virus type I dimerizes with transcription factors JunB and c-Jun and modulates their transcriptional activity." Basbous J., Arpin C., Gaudray G., Piechaczyk M., Devaux C., Mesnard J.M. J. Biol. Chem. 278:43620-43627(2003). Function: nan