Syncytin-2 (ERVFRD-1), partial, recombinant human

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 16-350aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: AYRHPDFPLL EKAQQLLQST GSPYSTNCWL CTSSSTETPG TAYPASPREW TSIEAELHIS YRWDPNLKGL MRPANSLLST VKQDFPDIRQ KPPIFGPIFT NINLMGIAPI CVMAKRKNGT NVGTLPSTVC NVTFTVDSNQ QTYQTYTHNQ FRHQPRFPKP PNITFPQGTL LDKSSRFCQG RPSSCSTRNF WFRPADYNQC LQISNLSSTA EWVLLDQTRN SLFWENKTKG ANQSQTPCVQ VLAGMTIATS YLGISAVSEF FGTSLTPLFH FHISTCLKTQ GAFYICGQSI HQCLPSNWTG TCTIGYVTPD IFIAPGNLSL PIPIYGNSPL PRVRR. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: This endogenous retroviral envelope protein has retained its original fusogenic properties and participates in trophoblast fusion and the formation of a syncytium during placenta morphogenesis. The interaction with MFSD2A is apparently important for this process. , Endogenous envelope proteins may have kept, lost or modified their original function during evolution but this one can still make pseudotypes with MLV, HIV-1 or SIV-1 virions and confer infectivity. Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. The surface protein mediates receptor recognition, while the transmembrane protein anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane. Reference: n/a. Function: nan