SUN domain-containing protein 3 (SUN3), partial, recombinant human

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 65-357aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: NHQWLKETDV PQKSRQLYAI IAEYGSRLYK YQARLRMPKE QLELLKKESQ NLENNFRQIL FLIEQIDVLK ALLRDMKDGM DNNHNWNTHG DPVEDPDHTE EVSNLVNYVL KKLREDQVEM ADYALKSAGA SIIEAGTSES YKNNKAKLYW HGIGFLNHEM PPDIILQPDV YPGKCWAFPG SQGHTLIKLA TKIIPTAVTM EHISEKVSPS GNISSAPKEF SVYGITKKCE GEEIFLGQFI YNKTGTTVQT FELQHAVSEY LLCVKLNIFS NWGHPKYTCL YRFRVHGTPG KHI. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: As a probable component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nuclear remodeling during sperm head formation in spermatogenesis. A probable SUN3:SYNE1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette. Reference: n/a. Function: nan