Succinyl-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial (OXCT1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 40-520aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: TKFYTDPVEA VKDIPDGATV LVGGFGLCGI PENLIDALLK TGVKGLTAVS NNAGVDNFGL GLLLRSKQIK RMVSSYVGEN AEFERQYLSG ELEVELTPQG TLAERIRAGG AGVPAFYTPT GYGTLVQEGG SPIKYNKDGS VAIASKPREV REFNGQHFIL EEAITGDFAL VKAWKADRAG NVIFRKSARN FNLPMCKAAE TTVVEVEEIV DIGAFAPEDI HIPQIYVHRL IKGEKYEKRI ERLSIRKEGD GEAKSAKPGD DVRERIIKRA ALEFEDGMYA NLGIGIPLLA SNFISPNITV HLQSENGVLG LGPYPRQHEA DADLINAGKE TVTILPGASF FSSDESFAMI RGGHVDLTML GAMQVSKYGD LANWMIPGKM VKGMGGAMDL VSSAKTKVVV TMEHSAKGNA HKIMEKCTLP LTGKQCVNRI ITEKAVFDVD KKKGLTLIEL WEGLTVDDVQ KSTGCDFAVS PKLMPMQQIA N. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate. Reference: Clinical and molecular characterization of five patients with succinyl-CoA:3-ketoacid CoA transferase (SCOT) deficiency.Fukao T., Sass J.O., Kursula P., Thimm E., Wendel U., Ficicioglu C., Monastiri K., Guffon N., Baric I., Zabot M.T., Kondo N.Biochim. Biophys. Acta 1812:619-624(2011). Function: Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.