Sterol carrier protein 2 (Scp2), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 1-547aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MPSVALNSPR LPRVFVVGVG MTKFMKPGGE NSRDYPDLAK EAGQKALADR QIPYSAVEQA CVGYVYGEST CGQRAIYHSL GLTGIPIINV NNNCSTGSTA LFMAQQLVQG GLANCVLALG FEKMEKGSLG TKYSDRSNPL EKHIDVLINK YGMSACPFAP QLFGSAGKEH METYGTKVEH FAKIGWKNHK HSVNNPYSQF QDEYSLDEIM KSRPVFDFLT VLQCCPTSDG AAAAIVSSEE FVQKHGLQSK AVEIVAQEMV TDMPSTFEEK SVIKMVGYDM SKEAARKCYE KSGLGPSDVD VIELHDCFST NELLTYEALG LCPEGQGGAL VDRGDNTYGG KWVINPSGGL ISKGHPLGAT GLAQCAELCW QLRGEAGKRQ VPGAKVALQH NLGLGGAAVV TLYRMGFPEA ASSFRTHQIS AAPTSSAGDG FKANLIFKEI EKKLEEEGEE FVKKIGGIFA FKVKDGPGGK EATWVVDVKN GKGSVLPDSD KKADCTITMA DSDLLALMTG KMNPQSAFFQ GKLKIAGNMG LAMKLQSLQL QPDKAKL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: [Isoform SCPx]: Plays a crucial role in the peroxisomal oxidation of branched-chain fatty acids. Catalyzes the last step of the peroxisomal beta-oxidation of branched chain fatty acids and the side chain of the bile acid intermediates di- and trihydroxycoprostanic acids (DHCA and THCA). Also active with medium and long straight chain 3-oxoacyl-CoAs. Stimulates the microsomal conversion of 7-dehydrocholesterol to cholesterol and transfers phosphatidylcholine and 7-dehydrocholesterol between membrances, in vitro. Isoforms SCP2 and SCPx cooperate in peroxisomal oxidation of certain naturally occurring tetramethyl-branched fatty acyl-CoAs., [Isoform SCP2]: Mediates the transfer of all common phospholipids, cholesterol and gangliosides from the endoplasmic reticulum to the plasma membrane. May play a role in regulating steroidogenesis. Stimulates the microsomal conversion of 7-dehydrocholesterol to cholesterol. Also binds fatty acids and fatty acyl Coenzyme A (CoA) such as phytanoyl-CoA. Involved in the regulation phospholipid synthesis in endoplasmic reticulum enhancing the incorporation of exogenous fatty acid into glycerides. Seems to stimulate the rate-limiting step in phosphatidic acid formation mediated by GPAT3. Isoforms SCP2 and SCPx cooperate in peroxisomal oxidation of certain naturally occurring tetramethyl-branched fatty acyl-CoAs. Reference: "Peroxisomal fatty acid oxidation disorders and 58 kDa sterol carrier protein X (SCPx). Activity measurements in liver and fibroblasts using a newly developed method." Ferdinandusse S., Denis S., van Berkel E., Dacremont G., Wanders R.J. J. Lipid Res. 41:336-342(2000). Function: nan