Speedy Ligase

Speedy Ligase is an improved DNA Ligase developed to carry out fast (less than 15 minutes) and efficient ligation of sticky or bluntend DNA at room temperature. The enzyme catalyses the formation of a phosphodiester bond between juxtaposed 5'-phosphoryl and 3'-hydroxyl termini in duplex DNA. Rapid ligation is based on the combination of Speedy Ligase with a 4x Speedy ligation buffer. Applications: - Cloning of both blunt- and sticky (cohesive)-end restriction fragments - Cloning of PCR products - Joining linkers and adapters to DNA - Nick repair - Self-circularization of linear DNA. Components: * Speedy Ligase (10 U/µL) * Reaction buffer (4x). Shipping Conditions: Dry Ice. Storage Conditions: -85 °C to -15 °C. Activity: Blunt-end ligation, Sticky-end ligation. Product Category: Ligases. Reaction Temperature: 16 - 25 0C. Heat Inactivation: 65 °C. Concentration: 10 U/µL