Sodium-dependent Multi-Vitamin Transporter, Rat, Control Peptide (SMVT)

A sodium-dependent transport system is responsible for transfer and distribution of vitamins to different parts of the body, the transfer includes vitamins like pantothenate, biotin, and ascorbic acid etc, These transporters belong to Solute Carrier family (SLC). Since vitamins are required for essential metabolic processes in all mammalian cells, such cells have developed intrinsic mechanisms to active accumulation of essential vitamins. Thus transporters help these cells to fulfill their requirement, they include Sodium-dependent Multi-Vitamin Transporter (SMVT), Sodium-dependent Vitamin-C Transporter (SVCT) 1 & 2, Creatine Transporter (CRT1/ CT1). The other vitamin transporters from SLC family include Thiamine Transporter Protein 1 (THTR1), Folate Transporter or Reduced Folate Carrier 1 (RFC1), Thyroid Iodide Transporter (TIT) and Taurine Transporter (TAU) etc. Sodium-dependent Multi-Vitamin Transporter (SMVT), a 635aa protein in rat and human (gene SLC5A6) is responsible for transplacental transfer of vitamins pantothenate, biotin and the essential metabolite lipoate. SMVT shows homology to other known sodium-dependent nutrient transporters, including bacterial pantothenate permease, mammalian iodide transporter, glucose transporter 1 & 2. Quantitatively, the absorptive tissues like the intestinal mucosa, kidney and placenta have very high amounts of SMVT-specific mRNA. Significant amount is also seen in liver, brain, and heart. Source: 22aa peptide from Rat SMVT, Location: ~N-terminal, Extracellular domain 1, Species Sequence Homology: Human-100%, Mouse-95%, Rabbit-80%. Applications: Suitable for use in ELISA as a control peptide for S5327-25. Other applications not tested. Recommended Dilution: ELISA: Coat ELISA plates at 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.