SMPD1 (human, recombinant)

Acid sphingomyelinase, also known as sphingomyelin phosphodiesterase 1 (SMPD1), is a lysosomal and secretory phosphodiesterase. SMPD1, the gene encoding acid sphingomyelinase, produces three isoforms but only the full-length isoform is further processed into a precursor polypeptide, which is composed of an N-terminal signal peptide, a saposin-like (SAP) domain, a proline-rich domain, a metallophosphoesterase catalytic region, and a C-terminal domain. The precursor polypeptide is subject to post-translational modifications and alternative trafficking, which results in two enzymes: lysosomal sphingomyelinase (L-SMase), which is further cleaved to an N-terminus of Gly66, and secretory sphingomyelinase (S-SMase), which is further cleaved to an N-terminus of His60. Both enzymes catalyze the hydrolysis of sphingomyelin into ceramide and phosphocholine. Acid sphingomyelinase is ubiquitously expressed and hydrolyzes sphingomyelin in the endo-lysosome, in lipoproteins, and at the outer leaflet of the plasma membrane. It has roles in various cellular processes, including apoptosis, immune cell activation, and inflammation. Mutations in SMPD1 result in type A and B Niemann-Pick disease, a lysosomal storage disorder characterized by sphingomyelin accumulation in the endo-lysosome and visceral, neurological, and psychiatric symptoms. Cayman's SMPD1 (human, recombinant) protein can be used for enzyme activity assay applications. This protein consists of 593 amino acids, has a calculated molecular weight of 66.3 kDa, and a predicted N-terminus of Leu47 after signal peptide cleavage.Synonyms: Acid Sphingomyelinase, ASM, aSMase, Sphingomyelin Phosphodiesterase 1, Zn-SMase. Purity: >90% as estimated by SDS-PAGE. Endotoxin Testing: <1.0 EU/µg determined by the LAL endotoxin assay. Source: Active recombinant human C-terminal His-tagged acid sphingomyelinase expressed in insect cells. Amino Acids: 47-628. MW: 66.3 kDa. Formulation: (Request formulation change), 20 mM Tris, 500 mM sodium chloride, with 25% glycerol, pH 7.5. UniProt Accession No: P17405.