Siglec 1 Fc Chimera, Recombinant, Mouse (Sialic acid binding Ig-like lectin 1, Sialoadhesin, CD169,

Siglecs are sialic acid specific I­t ype lectins that belong to the immunoglobulin superfamily. Structurally, they are transmembrane proteins with an N­terminal Ig ­like V­set domain followed by varying numbers of Ig­like C2­set domains (1, 2). Mouse Siglec­1, also known as sialoadhesin and CD169, is a 175 ­185kD glycoprotein that consists of a 1619 amino acid (aa) extracellular domain (ECD) with one Ig­like V­set domain and 16 Ig­like C2­set domains, a 21 aa transmembrane segment , and a 35 aa cytoplasmic domain (3, 4). Within the ECD, mouse Siglec­1 shares 73% and 83% aa sequence identity with human and rat Siglec­1, respectively. Alternate splicing generates a soluble form of the ECD and a soluble isoform that is truncated following the first three Ig­like domains (3). Siglec­1 expression is restricted to lymphnode and spleen macrophages and some tissue macrophages (4). The adhesive function of Siglec­1 is supported by the N­terminal Ig ­like domain which shows a selectivity for alpha­2, 3­linked sialic acid residues (4­6). Siglec­1 binds a number of sialylated molecules including the mannose receptor, MGL1, MUC1, PSGL­1, and different glycoforms of CD43 (7­10) . Its binding capacity can be masked by endogenous sialylated molecules (11, 12). The sialylated and sulfated N­linked carbohydrates that modify Siglec­1 itself are required for ligand binding (7,8). Siglec­1 is expressed on dendritic cells following rhinovirus exposure, and these DC promote T cell anergy (13). It is also induced on circulating monocytes during systemic sclerosis and HI V­1 infection (14­16). Siglec­1 can trap HI V­1 particles for trans infection of permissive cells (15). Source: Murine myeloma cell line, NS0-derived Mouse Siglec1 (Thr20 Arg1639) Accession# NP_035556, IEGRMDP, Mouse IgG2a (Glu98 Lys330) , N-terminal Sequence Analysis: Thr20, Structure: Disulfidelinked homodimer, Predicted Molecular Mass: 201.7kD (monomer) SDS-PAGE: 190-205kD under reducing conditions, Activity: Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The ED50 for this effect is typically ~0.6ug/ml. Endotoxin: <1.0 EU per 1ug of the protein by the LAL method. , Applications:, Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: 12 months from date of receipt, 20 to 70°C as supplied., 1 month, 2 to 8°C under sterile conditions after reconstitution., 3 months, 20 to 70°C under sterile conditions after reconstitution., Use a manual defrost freezer and avoid repeated freeze-thaw cycles.