SFRP2, Recombinant, Mouse (Secreted Frizzled Related Protein 2, SARP-1, SDF-5)

Secreted Frizzled Related Proteins (sFRPs) are a family of vertebrate proteins which contain homology to the ligand-binding domain of the Frizzled family of transmembrane receptors. The sFRPs are approximately 30-35kD in size and are comprised of 3 domains: a signal sequence, a cysteine-rich domain (CRD) of about 110 amino acids (aa) with high degree of similarity to the Frizzled proteins, including 10 conserved cysteines, and a 175aa conserved hydrophilic carboxy terminal region. Because sFRPs contain a CRD very similar to the region responsible for binding Wnt ligands in Frizzleds, sFRPS are thought to act as soluble antagonists of Wnt signals. sFRP-2, also known as SARP-1, SDF-5, and FRP-2, is expressed during mouse embryogenesis in the eye, brain, neural tube, craniofacial mesenchyme, joints, testis, pancreas, kidney and regions of smooth muscle cell development. Expression in the adult animal has been detected in the eye, heart, lung, along with preadipose and adipose tissues in mice and humans. Mouse and human sFRP-2 proteins share 98% amino acid identity, and related proteins have also been identified in chick, rabbit, and frog. A variety of activities have been reported for sFRP-2 including an increased resistance to apoptosis, antagonism of Xwnt -8 signaling in Xenopus, regulation of Wnt-4 signaling (with sFRP-1) in renal organogenesis, and inhibition of the migration of glioma cells (1-7). Source: A DNA sequence encoding the recombinant mouse secreted Frizzled Related Protein-2 was expressed with a C-terminal His tag in a mouse myeloma cell line, NS0. Molecular Mass: Based on N-terminal sequencing, mature rmsFRP-2 has Leu25 at the amino terminus. The monomeric rmsFRP-2 has a calculated mass of ~31.6kD. As a result of glycosylation the recombinant protein migrates with an apparent mass of approximately 35-40kD in SDS-PAGE under reducing conditions. Endotoxin Level: < 1.0EU per 1ug of the protein as determined by the LAL method. Activity: Measured by its ability to compete for the binding of rmFrizzled-1 and biotinylated rmWnt-3a. rmsFRPhas an IC50 value 0.7-3.5nM, under cconditions in which rmFrizzled-1/Fc is present at 5.4nM, and biotinylated rmWnt-3a concentration is 2.7nM. Reconstitution: Reconstitute with sterile PBS containing at least 0.1% HSA or BSA to prepare a stock solution of no less than 10ug/ml. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile PBS containing at least 0.1% HSA or BSA . Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.