Serpin B7 (SERPINB7), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-380aa. Protein Length: Full Length. Tag Info: N-terminal GST-tagged. Target Protein Sequence: MASLAAANAE FCFNLFREMD DNQGNGNVFF SSLSLFAALA LVRLGAQDDS LSQIDKLLHV NTASGYGNSS NSQSGLQSQL KRVFSDINAS HKDYDLSIVN GLFAEKVYGF HKDYIECAEK LYDAKVERVD FTNHLEDTRR NINKWVENET HGKIKNVIGE GGISSSAVMV LVNAVYFKGK WQSAFTKSET INCHFKSPKC SGKAVAMMHQ ERKFNLSVIE DPSMKILELR YNGGINMYVL LPENDLSEIE NKLTFQNLME WTNPRRMTSK YVEVFFPQFK IEKNYEMKQY LRALGLKDIF DESKADLSGI ASGGRLYISR MMHKSYIEVT EEGTEATAAT GSNIVEKQLP QSTLFRADHP FLFVIRKDDI ILFSGKVSCP. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Might function as an inhibitor of Lys-specific proteases. Might influence the maturation of megakaryocytes via its action as a serpin. Reference: "Mutations in SERPINB7, encoding a member of the serine protease inhibitor superfamily, cause Nagashima-type palmoplantar keratosis." Kubo A., Shiohama A., Sasaki T., Nakabayashi K., Kawasaki H., Atsugi T., Sato S., Shimizu A., Mikami S., Tanizaki H., Uchiyama M., Maeda T., Ito T., Sakabe J., Heike T., Okuyama T., Kosaki R., Kosaki K. Amagai M. Am. J. Hum. Genet. 93:945-956(2013). Function: nan