Serine--pyruvate aminotransferase, mitochondrial (Agxt), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 25-414aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: SYQLLVPPPE ALSKPLSVPT RLLLGPGPSN LAPRVLAAGS LRMIGHMQKE MLQIMEEIKQ GIQYVFQTRN PLTLVVSGSG HCAMETALFN LLEPGDSFLT GTNGIWGMRA AEIADRIGAR VHQMIKKPGE HYTLQEVEEG LAQHKPVLLF LVHGESSTGV VQPLDGFGEL CHRYQCLLLV DSVASLGGVP IYMDQQGIDI MYSSSQKVLN APPGISLISF NDKAKYKVYS RKTKPVSFYT DITYLAKLWG CEGETRVIHH TTPVTSLYCL RESLALIAEQ GLENCWRRHR EATAHLHKHL QEMGLKFFVK DPEIRLPTIT TVTVPAGYNW RDIVSYVLDH FSIEISGGLG PTEERVLRIG LLGYNATTEN VDRVAEALRE ALQHCPKNKL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Dual metabolic roles of gluconeogenesis (in the mitochondria) and glyoxylate detoxification (in the peroxisomes). Reference: SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways.Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.Mol. Cell 50:919-930(2013). Function: Dual metabolic roles of gluconeogenesis (in the mitochondria) and glyoxylate detoxification (in the peroxisomes).