Semenogelin-2 (SEMG2), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 24-582aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: QKGGSKGQLP SGSSQFPHGQ KGQHYFGQKD QQHTKSKGSF SIQHTYHVDI NDHDWTRKSQ QYDLNALHKA TKSKQHLGGS QQLLNYKQEG RDHDKSKGHF HMIVIHHKGG QAHHGTQNPS QDQGNSPSGK GLSSQCSNTE KRLWVHGLSK EQASASGAQK GRTQGGSQSS YVLQTEELVV NKQQRETKNS HQNKGHYQNV VDVREEHSSK LQTSLHPAHQ DRLQHGPKDI FTTQDELLVY NKNQHQTKNL SQDQEHGRKA HKISYPSSRT EERQLHHGEK SVQKDVSKGS ISIQTEEKIH GKSQNQVTIH SQDQEHGHKE NKISYQSSST EERHLNCGEK GIQKGVSKGS ISIQTEEQIH GKSQNQVRIP SQAQEYGHKE NKISYQSSST EERRLNSGEK DVQKGVSKGS ISIQTEEKIH GKSQNQVTIP SQDQEHGHKE NKMSYQSSST EERRLNYGGK STQKDVSQSS ISFQIEKLVE GKSQIQTPNP NQDQWSGQNA KGKSGQSADS KQDLLSHEQK GRYKQESSES HNIVITEHEV AQDDHLTQQY NEDRNPIST. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Participates in the formation of a gel matrix (sperm coagulum) entrapping the accessory gland secretions and ejaculated spermatozoa. Reference: "Molecular cloning of epididymal and seminal vesicular transcripts encoding a semenogelin-related protein."Lundwall A., Lilja H.Proc. Natl. Acad. Sci. U.S.A. 89:4559-4563(1992). Function: Participates in the formation of a gel matrix (sperm coagulum) entrapping the accessory gland secretions and ejaculated spermatozoa.