Semenogelin-1 (SEMG1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 24-402aa. Protein Length: Full Length of Isoform 2. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: QKGGSKGRLP SEFSQFPHGQ KGQHYSGQKG KQQTESKGSF SIQYTYHVDA NDHDQSRKSQ QYDLNALHKT TKSQRHLGGS QQLLHNKQEG RDHDKSKGHF HRVVIHHKGG KAHRGTQNPS QDQGNSPSGK GISSQYSNTE ERLWVHGLSK EQTSVSGAQK GRKQGGSQSS YVLQTEELVA NKQQRETKNS HQNKGHYQNV VEVREEHSSK VQTSLCPAHQ DKLQHGSKDI FSTQDELLVY NKNQHQTKNL NQDQQHGRKA NKISYQSSST EERRLHYGEN GVQKDVSQRS IYSQTEKLVA GKSQIQAPNP KQEPWHGENA KGESGQSTNR EQDLLSHEQK GRHQHGSHGG LDIVIIEQED DSDRHLAQHL NNDRNPLFT. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA. Reference: "Analysis of recombinant human semenogelin as an inhibitor of human sperm motility."Mitra A., Richardson R.T., O'Rand M.G.Biol. Reprod. 82:489-496(2010). Function: Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA.