Semaphorin-4C (Sema4c), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 21-664aa+IEGRMDP. Protein Length: Partial. Tag Info: C-terminal mFc-tagged. Target Protein Sequence: AEMWWNLVPR KTVSSGELVT VVRRFSQTGI QDFLTLTLTE HSGLLYVGAR EALFAFSVEA LELQGAISWE APAEKKIECT QKGKSNQTEC FNFIRFLQPY NSSHLYVCGT YAFQPKCTYI NMLTFTLDRA EFEDGKGKCP YDPAKGHTGL LVDGELYSAT LNNFLGTEPV ILRYMGTHHS IKTEYLAFWL NEPHFVGSAF VPESVGSFTG DDDKIYFFFS ERAVEYDCYS EQVVARVARV CKGDMGGART LQKKWTTFLK ARLVCSAPDW KVYFNQLKAV HTLRGASWHN TTFFGVFQAR WGDMDLSAVC EYQLEQIQQV FEGPYKEYSE QAQKWARYTD PVPSPRPGSC INNWHRDNGY TSSLELPDNT LNFIKKHPLM EDQVKPRLGR PLLVKKNTNF THVVADRVPG LDGATYTVLF IGTGDGWLLK AVSLGPWIHM VEELQVFDQE PVESLVLSQS KKVLFAGSRS QLVQLSLADC TKYRFCVDCV LARDPYCAWN VNTSRCVATT S. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration. Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade. Reference: "Semaphorin 4C and 4G are ligands of Plexin-B2 required in cerebellar development." Maier V., Jolicoeur C., Rayburn H., Takegahara N., Kumanogoh A., Kikutani H., Tessier-Lavigne M., Wurst W., Friedel R.H. Mol. Cell. Neurosci. 46:419-431(2011). Function: nan