Secretin receptor (SCTR), recombinant human

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 23-440aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: HSTGALPRLC DVLQVLWEEQ DQCLQELSRE QTGDLGTEQP VPGCEGMWDN ISCWPSSVPG RMVEVECPRF LRMLTSRNGS LFRNCTQDGW SETFPRPNLA CGVNVNDSSN EKRHSYLLKL KVMYTVGYSS SLVMLLVALG ILCAFRRLHC TRNYIHMHLF VSFILRALSN FIKDAVLFSS DDVTYCDAHR AGCKLVMVLF QYCIMANYSW LLVEGLYLHT LLAISFFSER KYLQGFVAFG WGSPAIFVAL WAIARHFLED VGCWDINANA SIWWIIRGPV ILSILINFIL FINILRILMR KLRTQETRGN EVSHYKRLAR STLLLIPLFG IHYIVFAFSP EDAMEIQLFF ELALGSFQGL VVAVLYCFLN GEVQLEVQKK WQQWHLREFP LHPVASFSNS TKASHLEQSQ GTCRTSII. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Receptor for secretin (SCT), which is involved in different processes such as regulation of the pH of the duodenal content, food intake and water homeostasis. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Upon binding to secretin, regulates the pH of the duodenum by (1) inhibiting the secretion of gastric acid from the parietal cells of the stomach and (2) stimulating the production of bicarbonate (NaHCO(3)) from the ductal cells of the pancreas. In addition to regulating the pH of the duodenal content, plays a central role in diet induced thermogenesis: acts as a non-sympathetic brown fat (BAT) activator mediating prandial thermogenesis, which consequentially induces satiation. Mechanistically, secretin released by the gut after a meal binds to secretin receptor (SCTR) in brown adipocytes, activating brown fat thermogenesis by stimulating lipolysis, which is sensed in the brain and promotes satiation. Also able to stimulate lipolysis in white adipocytes. Also plays an important role in cellular osmoregulation by regulating renal water reabsorption. Also plays a role in the central nervous system: required for synaptic plasticity. Reference: n/a. Function: nan