Roundabout homolog 1 (Robo1), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 880-1612aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-Myc-tagged. Target Protein Sequence: YRHRKKRNGL TSTYAGIRKV PSFTFTPTVT YQRGGEAVSS GGRPGLLNIS EPATQPWLAD TWPNTGNNHN DCSINCCTAG NGNSDSNLTT YSRPADCIAN YNNQLDNKQT NLMLPESTVY GDVDLSNKIN EMKTFNSPNL KDGRFVNPSG QPTPYATTQL IQANLSNNMN NGAGDSSEKH WKPPGQQKPE VAPIQYNIME QNKLNKDYRA NDTIPPTIPY NQSYDQNTGG SYNSSDRGSS TSGSQGHKKG ARTPKAPKQG GMNWADLLPP PPAHPPPHSN SEEYNMSVDE SYDQEMPCPV PPAPMYLQQD ELQEEEDERG PTPPVRGAAS SPAAVSYSHQ STATLTPSPQ EELQPMLQDC PEDLGHMPHP PDRRRQPVSP PPPPRPISPP HTYGYISGPL VSDMDTDAPE EEEDEADMEV AKMQTRRLLL RGLEQTPASS VGDLESSVTG SMINGWGSAS EEDNISSGRS SVSSSDGSFF TDADFAQAVA AAAEYAGLKV ARRQMQDAAG RRHFHASQCP RPTSPVSTDS NMSAVVIQKA RPAKKQKHQP GHLRREAYAD DLPPPPVPPP AIKSPTVQSK AQLEVRPVMV PKLASIEART DRSSDRKGGS YKGREALDGR QVTDLRTNPS DPREAQEQPN DGKGRGTRQP KRDLPPAKTH LGQEDILPYC RPTFPTSNNP RDPSSSSSMS SRGSGSRQRE QANVGRRNMA EMQVLGGFER GDENNEELEE TES. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed:10433822, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). May be required for lung development (PubMed:11734623). Reference: "The mouse homologue of human DUTT1/H-robo1 gene: protein sequence and chromosomal location."Wu M.C., Lowe N., Fordham R., Rabbitts P.Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases. Function: Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development