RNase III

Product Description: RNase III is a double-stranded RNA (dsRNA) -specific endoribonuclease that can cleave dsRNA into 18-25 bp interfering RNAs (siRNA) with 2 nucleotide 3' overhangs, 5' phosphate and 3' hydroxyl. RNase III can be used to generate siRNA library from long dsRNA for gene silencing and target validation, RNase III can also be used to remove dsRNA, a side product with immunogenicity, that is generated during in vitro transcription. Applications: Making siRNA library for any target gene, Gene silencing and target validation, Removal of dsRNA from in vitro transcription. Concentration: 2U/µL. Unit Definition: One unit (U) is defined as the amount of enzyme required to digest 1µg dsRNA into siRNA at 37°C in a 50 µL reaction mixture for 20 minutes.