Rhamnogalacturonan endolyase 9B, Bacteroides thetaiotaomicron

Rhamnogalacturonan endolyase 9B (BtRge9B), assigned the E.C. number 4.2.2.23, is a derivative of Bacteroides thetaiotaomicron. It is an enzyme that participates in the endotype eliminative cleavage of L-alpha-rhamnopyranosyl-1,4-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end. The recombinant BtRge9B, purified from Escherichia coli, is a single-domain Pectate Lyase family 9 (PL9) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Polysaccharide Lyase. Activity: Rhamnogalacturonan lyases. Family: PL9. Ec Number: 4.2.2.23. Organism: Bacteroides thetaiotaomicron. Architecture: PL9. Optimal pH: 7.5. Optimal Temperature: 37°C. Substrates: rhamnogalacturonan I