RG galacturonohydrolase 28A, Bacteroides thetaiotaomicron

RG galacturonohydrolase 28A (BtRgg28A), assigned the E.C. number 3.2.1.173, is a derivative of Bacteroides thetaiotaomicron. It is an enzyme that participates in the exohydrolysis of the alpha-D-GalA-1,2-alpha-L-Rha bond in rhamnogalacturonan oligosaccharides with initial inversion of configuration releasing D-galacturonic acid from the non-reducing end of rhamnogalacturonan oligosaccharides. The recombinant BtRgg28A, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 28 (GH28) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Glycoside Hydrolase. Activity: Galacturonohydrolases. Family: GH28. Ec Number: 3.2.1.173. Organism: Bacteroides thetaiotaomicron. Architecture: GH28. Optimal pH: 7. Optimal Temperature: 37°C. Substrates: rhamnogalacturonan I