Retinol-binding protein 3 (RBP3), partial, bovine, recombinant

Organism: Bos taurus (Bovine). Source: E.coli. Expression Region: 933-1231aa. Protein Length: Partial. Tag Info: N-terminal GST-tagged. Target Protein Sequence: AKVPTVLQTA GKLVADNYAS PELGVKMAAE LSGLQSRYAR VTSEAALAEL LQADLQVLSG DPHLKTAHIP EDAKDRIPGI VPMQIPSPEV FEDLIKFSFH TNVLEGNVGY LRFDMFGDCE LLTQVSELLV EHVWKKIVHT DALIVDMRFN IGGPTSSISA LCSYFFDEGP PILLDKIYNR PNNSVSELWT LSQLEGERYG SKKSMVILTS TLTAGAAEEF TYIMKRLGRA LVIGEVTSGG CQPPQTYHVD DTDLYLTIPT ARSVGAADGS SWEGVGVVPD VAVPAEAALT RAQEMLQHT. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina. Reference: Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method.Oshikawa M., Tsutsui C., Ikegami T., Fuchida Y., Matsubara M., Toyama S., Usami R., Ohtoko K., Kato S.Invest. Ophthalmol. Vis. Sci. 52:6662-6670(2011). Function: IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina.