Reducing-end cellobiohydrolase 48A, Thermobifida fusca

Reducing-end cellobiohydrolase 48A (TfCbh48A), assigned the E.C. number 3.2.1.176, is a derivative of Thermobifida fusca. It is an exo-1,4-beta-glucanse that acts by releasin cellobiose from the reducing ends of the cellulose chains. The recombinant TfCbh48A, purified from Escherichia coli, is a modular Glycoside Hydrolase family 48 (GH48) enzyme, with an N-terminal family 2 Carbohydrate Binding Module (CBM2) (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Glycoside Hydrolase. Activity: Cellobiohydrolases. Family: GH48. Ec Number: 3.2.1.176. Organism: Thermobifida fusca. Architecture: CBM2-GH48. Optimal pH: 5.5. Optimal Temperature: 50°C. Substrates: crystalline cellulose