Recombinant DDX41 Enzyme, Active, Human

Active, human full-length DDX41 (also known as ABS, MPLPF, or DEAD-box helicase 41) protein expressed and purified from Baculovirus. For specific activity, please refer to the Certificate of Analysis for each individual enzyme lot. Purity is >70% in a composition of 50 mM Tris HCl, 200 mM NaCl, 0.5 mM TCEP, and 10% Glycerol (pH 7.5). Currently used with the Transcreener assay at a concentration of 147.4 nM in a 10 µL reaction. Protein Function: Multifunctional protein that participates in many aspects of cellular RNA metabolism. Plays pivotal roles in innate immune sensing and hematopoietic homeostasis (PubMed:34473945). Recognizes foreign or self-nucleic acids generated during microbial infection, thereby initiating anti-pathogen responses (PubMed:23222971). Mechanistically, phosphorylation by BTK allows binding to dsDNA leading to interaction with STING1 (PubMed:25704810). Modulates the homeostasis of dsDNA through its ATP-dependent DNA-unwinding activity and ATP-independent strand-annealing activity (PubMed:35613581). In turn, induces STING1-mediated type I interferon and cytokine responses to DNA and DNA viruses (PubMed:35613581). Selectively modulates the transcription of certain immunity-associated genes by regulating their alternative splicing (PubMed:33650667). Binds to RNA (R)-loops, structures consisting of DNA/RNA hybrids and a displaced strand of DNA that occur during transcription, and prevents their accumulation, thereby maintaining genome stability (PubMed:36229594). Also participates in pre-mRNA splicing, translational regulation and snoRNA processing, which is essential for ribosome biogenesis (PubMed:36229594, PubMed:36780110) [The Uniprot Consortium]