Recombinant CD38 Enzyme, Active, Human

Active, human Cluster of Differentiation 38 enzyme, His-tagged protein expressed and purified from baculovirus-infected insect cells. The enzyme has been thoroughly validated with Transcreener ADPR Assay Kit. For specific activity, please refer to the Certificate of Analysis for each individual enzyme lot. Purity is >90% in a 50 mM MES buffer (pH 5.0), 100 mM NaCl, and 10% glycerol composition. Currently used with the Transcreener assay at a concentration between 0.0028 - 0.0103 nM in a 10 µL reaction. Protein Function: Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion (PubMed:7961800, PubMed:8253715). Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+) (PubMed:16690024). Has cADPR hydrolase activity (PubMed:7961800, PubMed:8253715) [The Uniprot Consortium]