Rabankyrin-5 (human, recombinant)

Rabankyrin-5 is a Rab5 effector protein with roles in endosomal fusion, micropinocytosis, and mitochondrial homeostasis and dynamics.1,2 It is composed of an N-terminal BTB domain, 21 ankyrin (ANK) repeats, and a C-terminal FYVE finger domain and primarily localizes to endosomal membranes, but can also localize to the cytosol.1 Rabankyrin-5 localizes with Rab5-positive early endosomes, and immunodepletion of Rabankyrin-5 using anti-Rabankyrin-5 antibodies inhibits early endosome fusion in HeLa cell cytosol. Knockdown of ANKFY1, the gene encoding Rabankyrin-5, using endoribonuclease-prepared small interfering RNA (esiRNA) inhibits EGF-induced micropinocytosis in skin cancer cells. ANKFY1 knockdown also induces mitochondrial elongation and stasis in retinal pigment epithelial (RPE) cells.3 Cayman's Rabankyrin-5 (human, recombinant) protein has a calculated molecular weight of 128.4 kDa.