Protein phosphatase 1 regulatory subunit 15A (PPP1R15A), partial, human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 40-674aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: GPLEPWLVEA VKGAALVEAG LEGEARTPLA IPHTPWGRRP EEEAEDSGGP GEDRETLGLK TSSSLPEAWG LLDDDDGMYG EREATSVPRG QGSQFADGQR APLSPSLLIR TLQGSDKNPG EEKAEEEGVA EEEGVNKFSY PPSHRECCPA VEEEDDEEAV KKEAHRTSTS ALSPGSKPST WVSCPGEEEN QATEDKRTER SKGARKTSVS PRSSGSDPRS WEYRSGEASE EKEEKAHKET GKGEAAPGPQ SSAPAQRPQL KSWWCQPSDE EEGEVKALGA AEKDGEAECP PCIPPPSAFL KAWVYWPGED TEEEEDEEED EDSDSGSDEE EGEAEASSST PATGVFLKSW VYQPGEDTEE EEDEDSDTGS AEDEREAETS ASTPPASAFL KAWVYRPGED TEEEEDEDVD SEDKEDDSEA ALGEAESDPH PSHPDQRAHF RGWGYRPGKE TEEEEAAEDW GEAEPCPFRV AIYVPGEKPP PPWAPPRLPL RLQRRLKRPE TPTHDPDPET PLKARKVRFS EKVTVHFLAV WAGPAQAARQ GPWEQLARDR SRFARRITQA QEELSPCLTP AARARAWARL RNPPLAPIPA LTQTLPSSSV PSSPVQTTPL SQAVATPSRS SAAAAAALDL SGRRG. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Reference: "The gadd and MyD genes define a novel set of mammalian genes encoding acidic proteins that synergistically suppress cell growth."Zhan Q., Lord K.A., Alamo I. Jr., Hollander M.C., Carrier F., Ron D., Kohn K.W., Hoffman B., Liebermann D.A., Fornace A.J. Jr.Mol. Cell. Biol. 14:2361-2371(1994). Function: Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.