Protein OPG161 (OPG161), partial, Variola virus, recombinant

Organism: Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus). Source: Mammalian cell. Expression Region: 57-184aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: VRLNQCMSAN EAAITDATAV AAALSTHRKV ASSTTQYKHQ ESCNGLYYQG SCYIFHSDYQ LFSDAKANCA TESSTLPNKS DVLTTWLIDY VEDTWGSDGN PITKTTTDYQ DSDVSQEVRK YFCVKTMN. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Forms a complex with OPG162 and OPG190 to coordinate the incorporation of OPG164 into wrapped enveloped virion (EV) membranes and, subsequently, the production of actin tails. Therefore plays an essential role in efficient cell-to-cell spread of viral particles. Reference: n/a. Function: nan