Protein kinase C-binding protein NELL1 (Nell1), recombinant rat

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 22-810aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: FGMDPDLQLD IISELDLVNT TLGVTQVAGL HNASKAFLFQ DVQREIHSAP HVSEKLIQLF RNKSEFTFLA TVQQKPSTSG VILSIRELEH SYFELESSGP REEIRYHYIH GGKPRTEALP YRMADGQWHK VALSVSASHL LLHIDCNRIY ERVIDPPETN LPPGSNLWLG QRNQKHGFFK GIIQDGKIIF MPNGFITQCP NLNRTCPTCS DFLSLVQGIM DLQELLAKMT AKLNYAETRL GQLENCHCEK TCQVSGLLYR DQDSWVDGDN CRNCTCKSGA VECRRMSCPP LNCSPDSLPV HISGQCCKVC RPKCIYGGKV LAEGQRILTK TCRECRGGVL VKITEACPPL NCSAKDHILP ENQCCRVCPG HNFCAEAPKC GENSECKNWN TKATCECKNG YISVQGNSAY CEDIDECAAK MHYCHANTVC VNLPGLYRCD CVPGYIRVDD FSCTEHDDCG SGQHNCDKNA ICTNTVQGHS CTCQPGYVGN GTICKAFCEE GCRYGGTCVA PNKCVCPSGF TGSHCEKDID ECAEGFVECH NHSRCVNLPG WYHCECRSGF HDDGTYSLSG ESCIDIDECA LRTHTCWNDS ACINLAGGFD CLCPSGPSCS GDCPHEGGLK HNGQVWILRE DRCSVCSCKD GKIFCRRTAC DCQNPNVDLF CCPECDTRVT SQCLDQSGQK LYRSGDNWTH SCQQCRCLEG EADCWPLACP SLGCEYTAMF EGECCPRCVS DPCLADNIAY DIRKTCLDSF GVSRLSGAVW TMAGSPCTTC KCKNGRVCCS VDLECIENN. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Plays a role in the control of cell growth and differentiation. Promotes osteoblast cell differentiation and terminal mineralization. Reference: n/a. Function: nan