Protein HAPLESS 2 (HAP2)-VLPs, recombinant Arabidopsis thaliana

Organism: Arabidopsis thaliana (Mouse-ear cress). Source: Mammalian cell. Expression Region: 25-705aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal Myc-tagged and C-terminal Flag-tagged (This tag can be tested only under denaturing conditions). Target Protein Sequence: IQILSKSKLE KCEKTSDSGN LNCSTKIVLN LAVPSGSSGG EASIVAEIVE VEDNSSSNMQ TVRIPPVITV NKSAAYALYD LTYIRDVPYK PQEYHVTTRK CEHDAGPDIV QICERLRDEK GNVLEQTQPI CCPCGPQRRM PSSCGDIFDK MIKGKANTAH CLRFPGDWFH VFGIGQRSLG FSVRVELKTG TRVSEVIIGP ENRTATANDN FLKVNLIGDF GGYTSIPSFE DFYLVIPREA AEAGQPGSLG ANYSMWMLLE RVRFTLDGLE CNKIGVGYEA FNTQPNFCSS PYWSCLHNQL WNFRESDINR IDRHQLPLYG LEGRFERINQ HPNAGPHSFS IGVTETLNTN LMIELRADDI EYVFQRSPGK IINIAIPTFE ALTQFGVAAV IIKNTGEVEA SYSLTFDCSK GVAFVEEQFF IIKPKAVTTR SFKLYPTKDQ AAKYICTAIL KDSQFSEVDR AECQFSTTAT VLDNGTQVTN PFQIPETQPK GFFDSIRILW TKIINGLVDF ITGDTCRNKC SSFFDFSCHI QYVCLSWMVM FGLLLALFPI TCLLLWLLHQ KGLFDPCYDW WEDHFDLDHH RRLLPSRADV VNRHHHHHKH RHHHNHHRRT HQRHKHHHGQ DDDVLQKMML ERDHSDSHYY HQLHRVHKDS KQKQRRRAKH GIVLPRDVHV ERQRKQRLRE S. Purity: The purity information is not available for VLPs proteins. Endotoxin: Not test. Biological Activity: n/a. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80°C. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20°C/-80°C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant). Relevance: Required for male fertility. Plays a role in pollen tube guidance and successful gamete attachment. Essential for the fusion of gametes during double fertilization, where one male gamete fuses with the egg to produce a zygote, and another male gamete fuses with the central cell to produce the endosperm. Mediates the fusion of cell membranes. Not required for pollen tube outgrowth. Reference: n/a. Function: nan