Protein FAM83D (Fam83d), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 1-585aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MAARFELLDD LPAACLSPCG PPNPTELFSE ARRLALEQLL AGGPDAWAAF LRRERLGRFL NADEVREVLG AAERPGEDGA AVAEDSFGSS HECSSGTYFP EQSDLEPPAL ELGWPSFYQG AYRGATRVEA HFQPRGAGAG GPYGCKDALR QQLRSAREVI AVVMDVFSDI DIFRDLQESC RKRGVAVYIL LDQTLLPHFL DMCMDLRVHP EQEKLMTVRT ITGNIYYARS GTKVVGKVHE KFTLIDGIRV ATGSYSFTWT DGKLNSSNLV ILSGQVVEHF DLEFRILYAQ SEPISSKLLS NFQINSKFDH LADRKPQSKE PTLGNLLRMR LARLSSTPRK SNLGPEEPPK DRAKPKRPDS EASTISDEDY FHSHKDQLED SKVADAATQT EPREEMAAVS LSEVGTQTSS SMMCVGTQTT VVTRAASSQA TVWSKSTTTQ TEADESFLPQ GAQSKEGSPA SKMSVSRSSS VRSSSSVSSQ GSLASSVSSH VSLTAADLHT PAYPKYLGLG TPHLDLCLRD SFRNLSKERQ VHFTGIRSRL TQMLTVLSRR TLFTEHYLSY SPGSFTRAST NLVSVRDIAL YPPYQ. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Required for proper chromosome congression and alignment during mitosis. Required for targeting KIF22/KID to the spindle microtubules. Reference: "The transcriptional landscape of the mammalian genome."Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005). Function: Probable proto-oncogene that regulates cell proliferation, growth, migration and epithelial to mesenchymal transition. Through the degradation of FBXW7, may act indirectly on the expression and downstream signaling of MTOR, JUN and MYC. May play also a role in cell proliferation through activation of the ERK1/ERK2 signaling cascade. May also be important for proper chromosome congression and alignment during mitosis through its interaction with KIF22.