Protein B5 (PS/HR), partial, Vaccinia virus, recombinant

Organism: Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)). Source: E.coli. Expression Region: 18-279aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: YSTCTVPTMN NAKLTSTETS FNDKQKVTFT CDQGYHSSDP NAVCETDKWK YENPCKKMCT VSDYISELYN KPLYEVNSTM TLSCNGETKY FRCEEKNGNT SWNDTVTCPN AECQPLQLEH GSCQPVKEKY SFGEYMTINC DVGYEVIGAS YISCTANSWN VIPSCQQKCD MPSLSNGLIS GSTFSIGGVI HLSCKSGFTL TGSPSSTCID GKWNPVLPIC VRTNEEFDPV DDGPDDETDL SKLSKDVVQY EQEIESLEAT YH. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Plays a role in the dissolution of the outermost membrane of extracellular enveloped virions to allow virion entry into host cells. Participates also in wrapping mature virions to form enveloped virions. Reference: "Acidic residues in the membrane-proximal stalk region of vaccinia virus protein B5 are required for glycosaminoglycan-mediated disruption of the extracellular enveloped virus outer membrane." Roberts K.L., Breiman A., Carter G.C., Ewles H.A., Hollinshead M., Law M., Smith G.L. J. Gen. Virol. 90:1582-1591(2009). Function: Plays a role in the dissolution of the outermost membrane of extracellular enveloped virions (EV) to allow virion entry into host cells. Participates also in wrapping mature virions (MV) to form enveloped virions (EV).