Protein atonal homolog 1 (Atoh1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 1-351aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MSRLLHAEEW AEVKELGDHH RHPQPHHVPP LTPQPPATLQ ARDLPVYPAE LSLLDSTDPR AWLTPTLQGL CTARAAQYLL HSPELGASEA AAPRDEADSQ GELVRRSGCG GLSKSPGPVK VREQLCKLKG GVVVDELGCS RQRAPSSKQV NGVQKQRRLA ANARERRRMH GLNHAFDQLR NVIPSFNNDK KLSKYETLQM AQIYINALSE LLQTPNVGEQ PPPPTASCKN DHHHLRTASS YEGGAGASAV AGAQPAPGGG PRPTPPGPCR TRFSGPASSG GYSVQLDALH FPAFEDRALT AMMAQKDLSP SLPGGILQPV QEDNSKTSPR SHRSDGEFSP HSHYSDSDEA S. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Transcriptional regulator. Activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. Plays a role in the differentiation of subsets of neural cells by activating E box-dependent transcription. Reference: "A mammalian helix-loop-helix factor structurally related to the product of Drosophila proneural gene atonal is a positive transcriptional regulator expressed in the developing nervous system." Akazawa C., Ishibashi M., Shimizu C., Nakanishi S., Kageyama R. J. Biol. Chem. 270:8730-8738(1995). Function: Transcriptional regulator. Activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. Plays a role in the differentiation of subsets of neural cells by activating E box-dependent transcription.