Probable E3 ubiquitin-protein ligase HERC1 (HERC1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 3975-4360aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: GMDEQIMSWA TSRPEDWHLG GKCDVYLWGA GRHGQLAEAG RNVMVPAAAP SFSQAQQVIC GQNCTFVIQA NGTVLACGEG SYGRLGQGNS DDLHVLTVIS ALQGFVVTQL VTSCGSDGHS MALTESGEVF SWGDGDYGKL GHGNSDRQRR PRQIEALQGE EVVQMSCGFK HSAVVTSDGK LFTFGNGDYG RLGLGNTSNK KLPERVTALE GYQIGQVACG LNHTLAVSAD GSMVWAFGDG DYGKLGLGNS TAKSSPQKID VLCGIGIKKV ACGTQFSVAL TKDGHVYTFG QDRLIGLPEG RARNHNRPQQ IPVLAGVIIE DVAVGAEHTL ALASNGDVYA WGSNSEGQLG LGHTNHVREP TLVTGLQGKN VRQISAGRCH SAAWTA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Involved in membrane trafficking via some guanine nucleotide exchange factor (GEF) activity and its ability to bind clathrin. Acts as a GEF for Arf and Rab, by exchanging bound GDP for free GTP. Binds phosphatidylinositol 4,5-bisphosphate, which is required for GEF activity. Reference: "p619, a giant protein related to the chromosome condensation regulator RCC1, stimulates guanine nucleotide exchange on ARF1 and Rab proteins." Rosa J.L., Casaroli-Marano R.P., Buckler A.J., Vilaro S., Barbacid M. EMBO J. 15:4262-4273(1996). Function: nan