Presenilins-associated rhomboid-like protein, mitochondrial (PARL), human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 53-379aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal Myc-tagged. Target Protein Sequence: FRKAPRKVEP RRSDPGTSGE AYKRSALIPP VEETVFYPSP YPIRSLIKPL FFTVGFTGCA FGSAAIWQYE SLKSRVQSYF DGIKADWLDS IRPQKEGDFR KEINKWWNNL SDGQRTVTGI IAANVLVFCL WRVPSLQRTM IRYFTSNPAS KVLCSPMLLS TFSHFSLFHM AANMYVLWSF SSSIVNILGQ EQFMAVYLSA GVISNFVSYV GKVATGRYGP SLGASGAIMT VLAAVCTKIP EGRLAIIFLP MFTFTAGNAL KAIIAMDTAG MILGWKFFDH AAHLGGALFG IWYVTYGHEL IWKNREPLVK IWHEIRTNGP KKGGGSK. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptotic signals. Required for the maturation of PINK1 into its 52kDa mature form after its cleavage by mitochondrial-processing peptidase (MPP) . Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain . Reference: "Mitochondrial processing peptidase regulates PINK1 processing, import and Parkin recruitment." Greene A.W., Grenier K., Aguileta M.A., Muise S., Farazifard R., Haque M.E., McBride H.M., Park D.S., Fon E.A. EMBO Rep. 13:378-385(2012). Function: nan