Plasma alpha-L-fucosidase (FUCA2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 29-467aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged and C-terminal 6xHis-tagged. Target Protein Sequence: HSATRFDPTW ESLDARQLPA WFDQAKFGIF IHWGVFSVPS FGSEWFWWYW QKEKIPKYVE FMKDNYPPSF KYEDFGPLFT AKFFNANQWA DIFQASGAKY IVLTSKHHEG FTLWGSEYSW NWNAIDEGPK RDIVKELEVA IRNRTDLRFG LYYSLFEWFH PLFLEDESSS FHKRQFPVSK TLPELYELVN NYQPEVLWSD GDGGAPDQYW NSTGFLAWLY NESPVRGTVV TNDRWGAGSI CKHGGFYTCS DRYNPGHLLP HKWENCMTID KLSWGYRREA GISDYLTIEE LVKQLVETVS CGGNLLMNIG PTLDGTISVV FEERLRQMGS WLKVNGEAIY ETHTWRSQND TVTPDVWYTS KPKEKLVYAI FLKWPTSGQL FLGHPKAILG ATEVKLLGHG QPLNWISLEQ NGIMVELPQL TIHQMPCKWG WALALTNVI. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. Reference: "Role for alpha-L-fucosidase in the control of Helicobacter pylori-infected gastric cancer cells." Liu T.W., Ho C.W., Huang H.H., Chang S.M., Popat S.D., Wang Y.T., Wu M.S., Chen Y.J., Lin C.H. Proc. Natl. Acad. Sci. U.S.A. 106:14581-14586(2009). Function: nan