Phosphoprotein associated with glycosphingolipid-enriched microdomains 1(PAG1), partial, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 38-432aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: SSCDREKKPR QHSGDHENLM NVPSDKEMFS RSVTSLATDA PASSEQNGAL TNGDILSEDS TLTCMQHYEE VQTSASDLLD SQDSTGKPKC HQSRELPRIP PESAVDTMLT ARSVDGDQGL GMEGPYEVLK DSSSQENMVE DCLYETVKEI KEVAAAAHLE KGHSGKAKST SASKELPGPQ TEGKAEFAEY ASVDRNKKCR QSVNVESILG NSCDPEEEAP PPVPVKLLDE NENLQEKEGG EAEESATDTT SETNKRFSSL SYKSREEDPT LTEEEISAMY SSVNKPGQLV NKSGQSLTVP ESTYTSIQGD PQRSPSSCND LYATVKDFEK TPNSTLPPAG RPSEEPEPDY EAIQTLNREE EKATLGTNGH HGLVPKENDY ESISDLQQGR DITRL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Negatively regulates TCR T-cell antigen receptor-mediated signaling in T-cells and FCER1 high affinity immunoglobulin epsilon receptor-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling. Reference: n/a. Function: nan