Phospholipase A2 group XV (PLA2G15), dog, recombinant

Organism: Canis lupus familiaris (Dog) (Canis familiaris). Source: Baculovirus. Expression Region: 32-408aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: RRPPVVLVPG DLGNQLEAKL DKPTVVHYLC SKRTESYFTL WLNLELLLPV IIDCWIDNIR LVYNRTSRAT QFPDGVDVRV PGFGKTFSLE FLDPSKSSVG SYFHTMVESL VDWGYIRGED VRGAPYDWRR APNENGPYFL ALREMIEEMY QLYGGPVVLV AHSMGNMYTL YFLQRQPQAW KNKYIQAFVA LGAPWGGVAK TLRVLASGDN NRIPVIRPLK IREQQRSAVS TSWLLPYNYT WSPEKIFVHT PTANYTLRDY HQFFQDIGFK DGWLMRQDTE GLVEAMVPPG VPLHCLYGTG VPTPDSFYYE SFPDRDPKIC FGDGDGTVNL QSALQCQAWR GHQEHQVSLQ ALPGSEHIEM LANATTLAYL KRVLLGP. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Has transacylase and calcium-independent phospholipase A2 activity. Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid. Reference: "Cloning and characterization of a lysosomal phospholipase A2, 1-O-acylceramide synthase." Hiraoka M., Abe A., Shayman J.A. J. Biol. Chem. 277:10090-10099(2002). Function: nan