PHD3, Human, Control Peptide (PH3, Prolyl Hydroxylase Domain-containing 3, HIF2PH3, HIF-Prolyl hydro

Oxygen is absolutely critical for the survival of mammalian cells. Hypoxia induced factor (HIF) is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. There are 3-types of alpha subunits (HIF1-3alpha) and one HIF-beta subunits. However, the three HIF-alpha subunits are regulated by oxygen in a similar fashion, i.e. by regulated stabilization of the alpha-subunits. Under normal conditions, HIF-alpha Prolines are hydroxylated at Pro-402 and Pro-564. This allows binding of von Hippel-Lindau (VHL), the substrate recognition component of the E3 ubiquinated ligase complex, subsequent ubiquitination and degradation of HIF-alpha by the proteasome. Under hypoxic conditions, hydroxylation of HIF-alpha is inhibited and this prevents HIF-alpha degradation. The enzymes responsible for HIF-hydroxylation are known as HIF-prolyl hydroxylases (PHD1-3 or HPH1, HPH2, and HPH3). The three PHDs have been identified to hydroxylate the motif, LXXLAP* with *P being the hydroxyproline. PHD3 (human 333-aa, chromosome 14, also known as EGL9, C. ELEGANS, HOMOLOG OF, 3, EGLN3, PROLYL HYDROXYLASE DOMAIN-CONTAINING PROTEIN 3,PHD3, HIF-PROLYL HYDROXYLASE 3, HIFPH3) Widely expressed at low levels. Expressed at higher levels in heart (cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle) and placenta. It is activated under hypoxia. Source: 16aa peptide of Human PHD3/EGLN3 (1), Location: ~C-terminal, Species Sequence Homology: Mouse, rat-87%. Applications: Suitable for use in ELISA as a control peptide for P3375-07. Other applications not tested. Recommended Dilution: ELISA: Use 50-100ng of control peptide/well, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.