Peroxisomal biogenesis factor 19 (PEX19) (Active), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-296aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal GST-tagged. Target Protein Sequence: AAAEEGCSVG AEADRELEEL LESALDDFDK AKPSPAPPST TTAPDASGPQ KRSPGDTAKD ALFASQEKFF QELFDSELAS QATAEFEKAM KELAEEEPHL VEQFQKLSEA AGRVGSDMTS QQEFTSCLKE TLSGLAKNAT DLQNSSMSEE ELTKAMEGLG MDEGDGEGNI LPIMQSIMQN LLSKDVLYPS LKEITEKYPE WLQSHRESLP PEQFEKYQEQ HSVMCKICEQ FEAETPTDSE TTQKARFEMV LDLMQQLQDL GHPPKELAGE MPPGLNFDLD ALNLSGPPGA SGEQC. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized ABCD1 at 5 µg/ml can bind human PEX19,the EC50 of human PEX19 protein is 22.96-33.00 µg/ml. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53. Reference: n/a. Function: nan