Peroxiredoxin II, active human recombinant protein

Peroxiredoxin 2, full length (Gene bank accession number NM_005809) with N-terminal HIS tag, MW=22.7 kDa, expressed in Baculovirus infected Sf9 cell expression system. 3.45 µg of PRDX2 was incubated in 50 mM Hepes (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30 °C for 5 min, followed by the addition of 0.22 mM H2O2. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min.